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Yuan Wen, Ivan Vechetti , Taylor Valentino, John J McCarthy 2020. High-Yield Skeletal Muscle Protein Recovery from TRIzol® after RNA and DNA Extraction. protocols.io dx.doi.org/10.17504/protocols.io.bii3kcgnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bii3kcgn
Authors: Yuan Wen, Ivan Vechetti , Taylor Valentino, John J McCarthy
Summary: Extraction of DNA, RNA, and protein from the same sample would allow for direct comparison of genomic, transcriptomic, and proteomic information. Commercially available kits exhibit poor protein yield and TRIzol® reagent produces a protein pellet that is extremely difficult to solubilize. In response to these limitations, this study presents an optimized method for the extraction of protein from the organic phase of TRIzol® reagent that allows for higher yield recovery of skeletal muscle protein compared to direct homogenization in a common protein lysis buffer. The presented method is inexpensive, simple and fast; requires no additional treatment of the protein pellet for dissolution; and is compatible with downstream western blot applications.
Affiliations: University of Kentucky, University of Kentucky, University of Kentucky, University of Kentucky
Version: 1
Publication Date: 2020
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Source: Protocols.io
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