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Carly Martin, Abdul Abdul, Charles Vanderburg, Naeem Nadaf, Ashley Feirrera, Evan Macosko 2020. Frozen Tissue Nuclei Extraction (for 10xV3 snSEQ). protocols.io dx.doi.org/10.17504/protocols.io.bi62khgeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bi62khge
Authors: Carly Martin, Abdul Abdul, Charles Vanderburg, Naeem Nadaf, Ashley Feirrera, Evan Macosko
Summary: Protocol for extraction of nuclei from frozen tissue in preparation for single-nuclei sequencing (droplet-based/10X). This protocol is based strongly on a similar extraction protocol from the McCarroll lab.
Affiliations: Broad Institute, Broad Institute, Broad Institute, Broad Institute, Broad Institute, Broad Institute
Version: 2
Publication Date: 2020
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Source: Protocols.io
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