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Oriol Pavón Arocas, Tiago Branco 2021. Preparation of acute midbrain slices for patch-clamp recordings. protocols.io dx.doi.org/10.17504/protocols.io.bhfzj3p6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bhfzj3p6
Authors: Oriol Pavón Arocas, Tiago Branco
Summary: Here we describe two different approaches to prepare acute midbrain slices from young adult mice for slice electrophysiology experiments. We also provide general advice for troubleshooting common issues leading to suboptimal slice quality, as well as some suggestions to ensure good maintenance of a patch-clamp rig.
Affiliations: Sainsbury Wellcome Centre for Neural Circuits and Behaviour, University College London, Howland Street, London, W1T 4JG, UK, Sainsbury Wellcome Centre for Neural Circuits and Behaviour, University College London, Howland Street, London, W1T 4JG, UK
Version: 1
Publication Date: 2021
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Source: Protocols.io
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