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URL: https://dx.doi.org/10.17504/protocols.io.bfayjifw

Authors: New England Biolabs

Group: New England Biolabs (NEB)

Summary: The NEBExpress MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein inE. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.ReliableE. coli expression: substantial yields (up to 100 mg/L)Fusion to MBP has been shown to enhance the solubility of proteins expressed inE. coli(1)Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target proteinGentle elution with maltose; no detergents or harsh denaturants required

Affiliations: New England Biolabs

External URL: https://www.neb.com/protocols/2020/02/05/nebexpress-mbp-fusion-and-purification-system-quick-start-protocol-neb-e8201

Version: 1

Publication Date: 2020

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