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Sarah Romac 2020. Nucleic acids extraction from single cell using MasterPure Complete DNA purification (Epicenter). protocols.io dx.doi.org/10.17504/protocols.io.bekbjcsnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bekbjcsn
Authors: Sarah Romac
Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff
Summary: Radiolaria are protists which can't be cultivated. These microoganisms have to be isolated by single-cell for genetic identification and it can be difficult to get clean DNA.Here we optimized a DNA extraction protocol from protist single-cell.It works very well on single-cell Radiolaria, Foraminifers, but also Ciliates, Dinoflagelletes, Diatoms.
Affiliations: UMR7144-Roscoff Marine Station
Version: 2
Publication Date: 2020
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Source: Protocols.io
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