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URL: https://dx.doi.org/10.17504/protocols.io.be9wjh7e

Authors: Adriana Lopes Dos Santos, Claude Lemieux, Monique Turmel

Summary: Method used to eliminate bacterial contamination of a marine micro-algal culture. Note, this method is not guaranteed to be 100% successful either due to the fact that the antibiotic combination is not lethal to the bacteria present or it is possible that the strain may not be capable of prolonged growth without the presence of bacteria. This method is based on that of Droop, adapted according to Andersen but using a modern antibiotic mix suggested by S. Slocombe (SAMS - Scottish Association for Marine Science). The method described here have been successfully used by Christian Jeanthon (ECOMAP) to produce axenic cultures of diatoms and E. huxleyi.1. Droop, M. R. A procedure for routine purification of algal cultures with antibiotics. Br. Phycol. Bull. 3, 295–297 (1967).2. Andersen, R. Algal Cultering Techniques. (Academic Press, 2005).

Affiliations: Asian School of the Environment, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore, Département de biochimie, de microbiologie et de bio-informatique, Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC G1V 0A6, Canada., Département de biochimie, de microbiologie et de bio-informatique, Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC G1V 0A6, Canada.

Version: 1

Publication Date: 2020

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