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Franchesca Farris, Marda Jorgensen, Jerelyn Nick, Jesus Peñaloza 2020. CODEX Acquisition Protocol. protocols.io dx.doi.org/10.17504/protocols.io.bdifi4bnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bdifi4bn
Authors: Franchesca Farris, Marda Jorgensen, Jerelyn Nick, Jesus Peñaloza
Group: Human BioMolecular Atlas Program (HuBMAP) Method Development Community
Summary: Multiplex imaging of lymph node, thymus and spleen is accomplished using the Akoya Biosciences CODEX system at the HubMAP Tissue Mapping Center at the University of Florida, TMC-UF.This protocol is an overview of the CODEX raw data acquisition process guided by the CODEX Instrument Manager (CIM) , which is part of the CODEX Software Suite. The CODEX Instrument Manager is necessary to perform CODEX experiments. It controls the fluidics of the CODEX instrument the integration and synchronization with the Keyence microscope, image data formatting and facilitates transfer of data to the analysis computer.For more information, consult the Akoya Biosciences CODEX User Manual - A.0.
Affiliations: University of Florida, University of Florida, University of Florida, University of Florida
Version: 1
Publication Date: 2020
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Source: Protocols.io
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