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Ran Zhou 2020. Preparation of single cell suspensions from human intestinal biopsies for single cell genomics applications. protocols.io dx.doi.org/10.17504/protocols.io.bde3i3gnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bde3i3gn
Authors: Ran Zhou
Group: Human Cell Atlas Method Development Community, Helmsley project_Basu lab
Summary: The protocol is adapted from Fujii's and Smillies's reports for single cell transcriptome analysis from human intestines. It provides details on acquirement of single cell suspension from epithelium and lamina propria. This methods is modified to generate appropriate meterials from patient's intestinal biopsies for sinlge-cell transcriptome and genomic applications.
Affiliations: University of Chicago
Version: 1
Publication Date: 2020
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Source: Protocols.io
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