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Protocol Name
Hydroxyl Radical-scavenging Activity
DOI:10.17504/protocols.io.bdazi2f6 RRID Copied  
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Jing Xu 2020. Hydroxyl Radical-scavenging Activity. protocols.io dx.doi.org/10.17504/protocols.io.bdazi2f6
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.bdazi2f6

Authors: Jing Xu

Summary: The scavenging of hydroxyl radicals were determined by Guo et al. The reactions were performed in 0.3 ml of 20 mM sodium salicylate, 2.0 ml of 1.5 nM FeSO4, 1.0 ml of samples, and 1.0 ml of 6 mM H2O2.The reaction mixture was incubated for 1 hat 37 °C. The absorbance was measured at 510 nm. The inhibition percent and 50% of absorbance(EC50)were calculated.

Associated Publications: Zhou J, Yang Q, Zhu X, Lin T, Hao D, Xu J (2020) Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components. PLoS ONE 15(6): e0234435. doi: 10.1371/journal.pone.0234435

Affiliations: Hainan University

External URL: https://doi.org/10.1371/journal.pone.0234435

Version: 1

Publication Date: 2020

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Source: Protocols.io