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Protocol Name
DOI:10.17504/protocols.io.bch2it8e RRID Copied  
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Teuta Pilizota, Ekaterina Krasnopeeva, Jerko Rosko 2021. BFM speed recording with back-focal-plane interferometry. protocols.io dx.doi.org/10.17504/protocols.io.bch2it8e
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.bch2it8e

Authors: Teuta Pilizota, Ekaterina Krasnopeeva, Jerko Rosko

Group: Teuta Pilizota

Summary: The speed of bacterial flagellar motor (BFM) is measured with back-focal-plane interferometry. Heavily attenuated optical trap (855 nm laser) is used to detect the rotation of a polystyrene bead attached to a truncated flagellar filament. Time course of the bead rotation is recorded with the position-sensitive detector.

Affiliations: The University of Edinburgh, The University of Edinburgh, The University of Edinburgh

External URL: http://[1] https://www.pnas.org/content/114/38/E7969, [2] https://www.cell.com/biophysj/fulltext/S0006-3495(19)30392-3

Version: 1

Publication Date: 2021

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Source: Protocols.io