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Franziska Aron, Gunnar Neumann, Guido Brandt 2020. Non-UDG treated double-stranded ancient DNA library preparation for Illumina sequencing . protocols.io dx.doi.org/10.17504/protocols.io.bakricv6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bakricv6
Authors: Franziska Aron, Gunnar Neumann, Guido Brandt
Group: WarinnerGroup, MPI-SHH Archaeogenetics
Summary: Protocol for the preparation of double-stranded genomic libraries for Illumina sequencing, optimised for ancient DNA (aDNA). This protocol generates adapter ligated DNA fragments that can be used in conjunction with downstream Indexing protocols.This protocol does not include UDG (USER) treatment, in order to retain molecular 'damage' in the form of deaminated cytosines characteristic of aDNA. This protocol is modified after Meyer & Kircher (2010) Cold Spring Harb. Protoc. (doi: 10.1101/pdb.prot5448).
Affiliations: Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Department of Archaeogenetics, Max Planck Institute for the Science of Human History
Version: 1
Publication Date: 2020
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Source: Protocols.io
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