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Byung Joon Seung 2020. Quantitative analysis method of mRNA expression using dual RNA in situ hybridization by comparison with housekeeping genes. protocols.io dx.doi.org/10.17504/protocols.io.badcia2wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.badcia2w
Authors: Byung Joon Seung
Summary: In retrospective formalin-fixed paraffin-embedded (FFPE) samples, RNA quality of FFPE tissues showed variation following the storage period and fixation process. So we suggest quantitative analysis by dual detection of target mRNA and housekeeping genes in one section and calculated the target gene/housekeeping gene ratio by two open-source image analysis programs. This assay potentially allows for reliable quantification of mRNA expression levels in retrospective FFPE samples.
Associated Publications: Seung B, Cho S, Kim S, Lim H, Sur J (2020) Quantitative analysis of HER2 mRNA expression by RNA in situ hybridization in canine mammary gland tumors: Comparison with immunohistochemistry analysis. PLoS ONE 15(2): e0229031. doi: 10.1371/journal.pone.0229031
Affiliations: Konkuk University
External URL: https://doi.org/10.1371/journal.pone.0229031
Version: 1
Publication Date: 2020
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Source: Protocols.io
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