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Eric Danner 2020. Tn5 Library Prep for Deep Sequencing Loci of CRISPR/Cas9 Edited Cells. Single gene specific primer amplification (UDiTaS protocol with alterations). protocols.io dx.doi.org/10.17504/protocols.io.7k2hkyeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.7k2hkye
Authors: Eric Danner
Summary: This is the Tn5 gDNA prep published in the supplementary file of the UDiTaS paper (https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4561-9).Usage: measuring chromosome fusions, InDels, mRNA splicing, structural variants, large resection, off target integration. -> Some of these DNA repair outcomes are demonstrated using such a sample preparation in this paper (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455975/ ). My protocol is a modified version of UDiTaS but with a few tweaks like using nested primers. You will need a protein facility to produce the recombinant Tn5.Software for analysis is on https://github.com/ericdanneror https://github.com/editasmedicine/uditas
Affiliations: Max Delbrück Center
Version: 1
Publication Date: 2020
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Source: Protocols.io
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