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Ana Cristina Jaramillo Madrid, Justin Ashworth 2019. Tranformation of Thalassiosira pseudonana via bacterial conjugation. protocols.io dx.doi.org/10.17504/protocols.io.7fghjjwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.7fghjjw
Authors: Ana Cristina Jaramillo Madrid, Justin Ashworth
Summary: This protocol has been successfully used to express nourseothricin resistance gene, mVenus fluorescence protein and other proteins related to triterpenoids production in Thalassiosira pseudonana (Tp) strain CCMP1335. The original protocol was published by Karas et al. (2015) where a detailed description of L1 medium and plates preparation is presented.
Affiliations: University of Technology Sydney, University of Technology Sydney
Version: 1
Publication Date: 2019
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Source: Protocols.io
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