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Junyan Qian 2019. Toehold testing/ PURE system protein production. protocols.io dx.doi.org/10.17504/protocols.io.73ehqjeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.73ehqje
Authors: Junyan Qian
Group: iGEM EPFL
Summary: Our Toehold switches are tested using NEB PURExpress and OnePot PURE system (our own cell-free protein expression system, search OnePot for detailed protocols). Our goal is to define a concentration of Toehold switch and trigger DNA sequence which has a high protein (sfGFP in our case )expression but low or no leakage at same time.
Affiliations: EPFL - EPF Lausanne
Version: 1
Publication Date: 2019
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Source: Protocols.io
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