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Protocol Name
Toehold switch assembly
DOI:10.17504/protocols.io.72bhqan RRID Copied  
PDF Report How to cite
Junyan Qian 2019. Toehold switch assembly. protocols.io dx.doi.org/10.17504/protocols.io.72bhqan
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.72bhqan

Authors: Junyan Qian

Group: iGEM EPFL

Summary: General toehold assemble method using NEB Q5 High-Fidelity 2x Master Mix. Here we took Boir Noir toeholds as an example, it has four candidates we wanted to test. In order to have a higher yield, run 5 reactions for each candidates.

Affiliations: EPFL

Version: 1

Publication Date: 2019

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Source: Protocols.io