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URL: https://dx.doi.org/10.17504/protocols.io.6zihf4e

Authors: Carmen Farid, Aisha A. Mahmoud, Hanaa M. Donia, Hafsa M. Ghatesh

Summary: Serum soluble CD200 levels in psoriasis patients was measured by ELISA (Human CD200 PicoKine™ ELISA Kit, Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0993)), and CD200R expression level on both peripheral blood monocytes and lymphocytes were measured by flowcytometry using human CD200 R1 conjugated antibody(Human CD200 R1 Fluorescein-conjugated Antibody, Bio-Techne,Minneapolis, Minnesota, USA, Catalog # FAB3414F)). Cells were analyzed on a FACSCalibur flow cytometer using CellQuest pro software (BD Biosciences, San Diego, CA, USA). Membrane fluorescence intensity was estimated and expressed as mean fluorescence intensity (MFI).

Associated Publications: Ismail AA, Donia HM, Ghatesh HM, Farid CI (2020) CD200/CD200 receptor axis in psoriasis vulgaris. PLoS ONE 15(3): e0230621. doi: 10.1371/journal.pone.0230621

Affiliations: Department of Dermatology, Venereology and Andrology; Faculty of Medicine; Alexandria University; Egypt. , Department of Dermatology, Venereology and Andrology; Faculty of Medicine; Alexandria University; Egypt. , Department of Clinical and Chemical pathology; Faculty of Medicine; Alexandria University; Alexandria; Egypt. , Department of Dermatology, Venereology and Andrology; Faculty of Medicine; Alexandria University; Alexandria; Egypt.

External URL: https://doi.org/10.1371/journal.pone.0230621

Version: 1

Publication Date: 2019

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