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URL: https://dx.doi.org/10.17504/protocols.io.6r3hd8n

Authors: Kristen Upton, Robyn T. Sussman, Khushbu Patel, Gregory P. Way, Rebecca N. Adams, Gregory I. Sacks, Rebecca N. Adams, Paolo Fortina, John M. Maris, Jo Lynne Rokita

Summary: This protocol explains the process of how we collected MYCN, MYC, and Histone ChIP-Seq data, as well as ATAC-Seq data for neuroblastoma cell lines. This protocol is comprised of three sections: Cell Growth and Expansion for care of neuroblastoma cell lines, ChIP-Seq protocol, and ATAC-Seq Protocol. Table 1, within the document, outlines which data was collected for each cell line.

Affiliations: Division of Oncology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA, Division of Oncology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA, Department of Bioinformatics and Health Informatics, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA, Genomics and Computational Biology Graduate Group, University of Pennsylvania, Philadelphia, PA, 19104, USA, Cancer Genomics and Bioinformatics Laboratory, Sidney Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA, Division of Oncology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA, Cancer Genomics and Bioinformatics Laboratory, Sidney Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA, Cancer Genomics and Bioinformatics Laboratory, Sidney Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA, Division of Oncology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA; Department of Pediatrics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA, Division of Oncology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA; Department of Bioinformatics and Health Informatics, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA; Center for Data-Driven Discovery in Biomedicine, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA

Version: 1

Publication Date: 2019

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