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Protocol Name
Streptavidin-CBD purification
DOI:10.17504/protocols.io.6e8hbhw RRID Copied  
PDF Report How to cite
Manuela De Las Casas 2019. Streptavidin-CBD purification. protocols.io dx.doi.org/10.17504/protocols.io.6e8hbhw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.6e8hbhw

Authors: Manuela De Las Casas

Group: AEGIS - Madrid iGEM 2019

Summary: The aim of this protocol is to obtain the purified streptavidin with cellulose binding domains on both sides from the transformed bacteria E. coli. Later on, this protein will be attached to a membrane as part of the detection system. The obtention of the proteins starts at this point with transformed bacteria (E. coli).The previous steps to obtain the transformed bacteria and further steps to use the purified protein do not correspond to this protocol.

Affiliations: Universidad Complutense de Madrid, AEGIS - Madrid iGEM 2019

Version: 1

Publication Date: 2019

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Source: Protocols.io