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Manuela De Las Casas 2019. Streptavidin-CBD purification. protocols.io dx.doi.org/10.17504/protocols.io.6e8hbhwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.6e8hbhw
Authors: Manuela De Las Casas
Group: AEGIS - Madrid iGEM 2019
Summary: The aim of this protocol is to obtain the purified streptavidin with cellulose binding domains on both sides from the transformed bacteria E. coli. Later on, this protein will be attached to a membrane as part of the detection system. The obtention of the proteins starts at this point with transformed bacteria (E. coli).The previous steps to obtain the transformed bacteria and further steps to use the purified protein do not correspond to this protocol.
Affiliations: Universidad Complutense de Madrid, AEGIS - Madrid iGEM 2019
Version: 1
Publication Date: 2019
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Source: Protocols.io