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Paul Rutten, Richard Tennant, Jacob Beal, Christopher Workman, Traci Haddock-Angelli, Natalie Farny, Vinoo Selvarajah 2019. Calibration Protocol - Conversion of OD600 to Colony Forming Units (CFUs). protocols.io dx.doi.org/10.17504/protocols.io.5gjg3unCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.5gjg3un
Authors: Paul Rutten, Richard Tennant, Jacob Beal, Christopher Workman, Traci Haddock-Angelli, Natalie Farny, Vinoo Selvarajah
Group: iGEM Measurement
Summary: This procedure can be used to calibrate OD600 to colony forming unit (CFU) counts, which are directly relatable to the cell concentration of the culture, i.e. viable cell counts per mL. This protocol assumes that 1 bacterial cell will give rise to 1 colony. For the CFU protocol, you will need to count colonies for your two Positive Control (BBa_I20270) cultures and your two Negative Control (BBa_R0040) cultures. Protocol based on this Yeast Plate Count Protocol.
Affiliations: iGEM Measurement Committee, iGEM Measurement Committee, iGEM Measurement Committee, Technical University of Denmark, iGEM, Worcester Polytechnic Institute, iGEM
External URL: https://2019.igem.org/Measurement
Version: 2
Publication Date: 2019
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Source: Protocols.io
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