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URL: https://dx.doi.org/10.17504/protocols.io.549g8z6

Authors: Jacob Beal, Traci Haddock-Angelli, Markus Gershater, Vishal Sanchania, Russell Buckley-Taylor, Geoff Baldwin, Natalie Farny, Richard Tennant, Paul Rutten

Group: iGEM Measurement

Summary: You will prepare a dilution series of ​monodisperse silica microspheres and measure the ​Abs​600 in your plate reader. The size and optical characteristics of these microspheres are similar to cells, and there is a known amount of particles per volume. This measurement will allow you to construct a standard curve of particle concentration which can be used to convert 600 nm absorbance measurements into an estimated equivalent number of cells.

Associated Publications: Fedorec AJ, Robinson CM, Wen KY, Barnes CP, FlopR: An Open Source Software Package for Calibration and Normalization of Plate Reader and Flow Cytometry Data. ACS Synthetic Biology 9(9). doi: 10.1021/acssynbio.0c00296

Affiliations: iGEM Measurement Committee, iGEM, Synthace, Synthace, iGEM Measurement Committee, Imperial College London, iGEM Measurement Committee, iGEM Measurement Committee, iGEM Measurement Committee

External URL: https://2019.igem.org/Measurement

Version: 2

Publication Date: 2019

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