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Michael Cheng, Xue-Zhong Ma, Chao Jiang, Ian McGilvray, Sonya Macparland 2020. Mouse 2- Step Collagenase Liver Perfusion protocol . protocols.io dx.doi.org/10.17504/protocols.io.53qg8mwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.53qg8mw
Authors: Michael Cheng, Xue-Zhong Ma, Chao Jiang, Ian McGilvray, Sonya Macparland
Summary: Analyzing the mouse liver requires optimal cell recovery from the tissue in terms of quality (viability) and quantity. Harsh dissociation methods can damage fragile cell populations, resulting in low viability and difficulty in cell culture. We have developed a gentle surgical and enzymatic technique based on the two-step collagenase perfusion protocol for single-cell analysis and primary cell culture.
Affiliations: University of Toronto, University of Toronto, University of Toronto, University of Toronto, University of Toronto
Version: 1
Publication Date: 2020
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Source: Protocols.io
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