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Deepak Nanjappa and Kathryn Coyne 2019. Glass bead transformation of Heterosigma akashiwo. protocols.io dx.doi.org/10.17504/protocols.io.4qhgvt6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.4qhgvt6
Authors: Deepak Nanjappa and Kathryn Coyne
Summary: This protocol was developed for the glass bead-mediated transformation of Heterosigma akashiwo. This species does not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have repeated this protocol with success and have been able to maintain transformants on selection medium for several months.
Affiliations: University of Delaware
Version: 3
Publication Date: 2019
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Source: Protocols.io
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