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Protocol Name
Electroporation of Heterosigma akashiwo
DOI:10.17504/protocols.io.4qggvtw RRID Copied  
PDF Report How to cite
Deepak Nanjappa and Kathryn Coyne 2019. Electroporation of Heterosigma akashiwo. protocols.io dx.doi.org/10.17504/protocols.io.4qggvtw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.4qggvtw

Authors: Deepak Nanjappa and Kathryn Coyne

Summary: This protocol was developed for transformation of Heterosigma akashiwo by electroporation. This species does not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have had limited success with this protocol, which may need to be further optimized.

Affiliations: University of Delaware

Version: 3

Publication Date: 2019

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Source: Protocols.io