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Protocol Name
DOI:10.17504/protocols.io.3s3gngn RRID Copied  
PDF Report How to cite
Eva Feldman 2019. Biochemical Measures of Neuropathy - Western Blot Stripping. protocols.io dx.doi.org/10.17504/protocols.io.3s3gngn
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.3s3gngn

Authors: Eva Feldman

Group: Diabetic Complications Consortium

Summary: Summary:Oxidative stress is highly correlated with the metabolic changes caused by hyperglycemia. Increased levels of glucose overload mitochondria and result in the production of reactive oxygen species (ROS). In addition, the flow of excess glucose through cellular pathways decreases the cell’s normal ability to detoxify ROS. As a result, the neurons and axons of the peripheral nervous system contain increased levels of ROS and decreased antioxidant capacity. The following assays are used to measure these changes in rodent models of diabetic neuropathy. Diabetic Complication:

Affiliations: University of Michigan - Ann Arbor

External URL: https://www.diacomp.org/shared/document.aspx?id=54&docType=Protocol

Version: 1

Publication Date: 2019

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Source: Protocols.io