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Eva Feldman 2019. Biochemical Measures of Neuropathy - Western Blot Stripping. protocols.io dx.doi.org/10.17504/protocols.io.3s3gngnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.3s3gngn
Authors: Eva Feldman
Group: Diabetic Complications Consortium
Summary: Summary:Oxidative stress is highly correlated with the metabolic changes caused by hyperglycemia. Increased levels of glucose overload mitochondria and result in the production of reactive oxygen species (ROS). In addition, the flow of excess glucose through cellular pathways decreases the cell’s normal ability to detoxify ROS. As a result, the neurons and axons of the peripheral nervous system contain increased levels of ROS and decreased antioxidant capacity. The following assays are used to measure these changes in rodent models of diabetic neuropathy. Diabetic Complication:
Affiliations: University of Michigan - Ann Arbor
External URL: https://www.diacomp.org/shared/document.aspx?id=54&docType=Protocol
Version: 1
Publication Date: 2019
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Source: Protocols.io