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Protocol Name
DOI:10.17504/protocols.io.36fgrbn RRID Copied  
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Stephen Floor 2019. Ethanol precipitation of RNA from small or large volumes. protocols.io dx.doi.org/10.17504/protocols.io.36fgrbn
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.36fgrbn

Authors: Stephen Floor

Group: Stephen Floor Lab

Summary: How to precipitate RNA with ethanol and resuspend it! Two separate protocols - one for small and one for large volumes. The procedure for large volume precipitation is similar, but begins with an initial precipitation and transfers to a small tube for the final steps to avoid loss in the large tube. Strongly recommended to move to a smaller tube. Recommended reading: Walker & Lorsch RNA purification--precipitation methods.

Affiliations: UCSF

Version: 1

Publication Date: 2019

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Source: Protocols.io