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Protocol Name
DOI:10.17504/protocols.io.2xkgfkw RRID Copied  
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Renuka Kudva 2019. Preparation of a cell-free expression system from Escherichia coli. protocols.io dx.doi.org/10.17504/protocols.io.2xkgfkw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.2xkgfkw

Authors: Renuka Kudva

Summary: This protocol describes the procedure make an S30 derived E.coli cell lysate that lacks membrane components. Modified from a protocol published in https://doi.org/10.1091/mbc.e11-07-0590The procedure takes three days in total, including preparation time.

Affiliations: Stockholm University

Version: 1

Publication Date: 2019

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Source: Protocols.io