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URL: http://www.wormbase.org/db/get?name=WBStrain00054680
Proper Citation: RRID:WB-STRAIN:WBStrain00054680
Description: Caenorhabditis elegans with name cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III. from WB.
Species: Caenorhabditis elegans
Synonyms: cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III.
Notes: Made_by: Dmytro Kukhtar|"Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4."
Affected Gene: WBGene00003230(mex-5)|WBGene00004896(smu-2)|WBGene00006843(unc-119)
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No rating or validation information has been found for cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III..
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Source: Integrated Animals
Source Database: WormBase (WB)