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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD01813
Description: Tetrahymena thermophila with name BMCG-hy3-63 from TSC.
Species: Tetrahymena thermophila
Notes: major h3, h4 germline KO cells were rescued with hy3. Hybrid H3 contruct with H3(1-75) H3.3 (76-90) H3 (91-135), then transformed with Bsr-mtt1-cna1-gfp construct. CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cas. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: CNA1 (TTHERM_00146340), HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center
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