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URL: http://www.wormbase.org/db/get?name=WBStrain00054750
Proper Citation: RRID:WB-STRAIN:WBStrain00054750
Description: Caenorhabditis elegans with name pqe-1(utx65[mScarlet-I-C1::3xMyc::pqe-1]) III. from WB.
Species: Caenorhabditis elegans
Synonyms: pqe-1(utx65[mScarlet-I-C1::3xMyc::pqe-1]) III.
Notes: Made_by: Ann Houghton (Glow Worms '22)|"N-terminal tag of PQE-1 via CRISPR/Cas9 knock-in of mScarlet at pqe-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd 5 CCAGATGCGAAAGCGAACAG 3 ; rev 5 TGTACTTACCGGAATCGGCG 3. Left flank: 5' ttaataatcattccagcaagtatctcagcc 3'; Right flank: 5' ATGTTTAATGGCGGCTATGGATCTGGAAAC 3; sgRNA: atctcagccATGTTTAATGG; Cas9/sgRNA plasmid: pGLOW143; mScarlet^SEC^3xMyc plasmid: pGLOW142; SEC insertion allele strain: GLW84."
Affected Gene: WBGene00004095(pqe-1)
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No rating or validation information has been found for pqe-1(utx65[mScarlet-I-C1::3xMyc::pqe-1]) III..
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Source: Integrated Animals
Source Database: WormBase (WB)
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