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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=632518356
Proper Citation: RRID:RGD_632518356
Description: Rattus norvegicus with name SD-Dbhem1(cre)Xyan from RGD.
Species: Rattus norvegicus
Notes: DBH-Cre knock-in (KI) rats, developed on the Sprague Dawley (SD) rat background, were generated using the CRISPR/Cas9 system. In this knock-in model, a gene cassette encod- ing 2A peptide, fused to Cre recombinase was introduced to replace the TAA stop codon in exon 12 of the rat Dbh gene. Additionally, a synonymous mutation p. T616= (ACG to ACA) was incorporated to pre- vent gRNA binding and recutting of the sequence following homology-directed repair. The gRNA targeting the rat DBH gene (gRNA-B1: 5′-TTACTCAGTGTCTGCCTCCGTGG-3′), the donor vector con- taining the “P2A-Cre” cassette, and a synonymous mutation p. T616= (ACG to ACA), along with Cas9 mRNA, were co-injected into fertilized rat embryos. F0 founders. DBH-Cre KI rats were bred with wild-type SD rats (Guangdong Vital River Laboratory Animal Technology Co., Ltd., China). Shenzhen Institute of Advanced Technolog, Shenzhen, China
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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