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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=630350396
Proper Citation: RRID:RGD_630350396
Description: Rattus norvegicus with name SD-Dmdem1Ins from RGD.
Species: Rattus norvegicus
Notes: Deletion of exons 45 to 47 of the rat Dmd gene by CRISPR/Cas9 was performed by using two pairs of sgRNAs on both sides of exons 45 and 47. The spCas9 and sgRNAs were electroporated in rat Sprague Dawley (RjHan:SD) fertilized oocytes. Genotyping PCR and sequencing were used to confirm the deletion of exons 45–47 in F0 founder animals. Rats can be obtained through material transfer agreement by contacting the corresponding author at valentina.- [email protected] and [email protected].
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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