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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=626419677
Proper Citation: RRID:RGD_626419677
Description: Rattus norvegicus with name SD-Gnalem2Hpng from RGD.
Species: Rattus norvegicus
Notes: Gnal knockout rats were generated by CRISPR/Cas9 technology. Exon1 of rat Gnal splicing variant 2 was targeted, resulting in a deletion of 1 base pair that corresponds to position 44 downstream of the translation start point ATG of the Gnal splicing variant 2. CRISPR technology details: self-synthesized Cas9 mRNA using px 330 vector, applying T7 transcription kit followed by Poly-Adenylation and Capping. No sequencing performed, whether Cas9 has been inserted into the rat genome is unknown. Homozygous: Approximately 25% body weight reduction; Smaller body size; Hyperactivity; Better rotarod performance than wild-type littermates.Heterozygous: No body weight change; No body size change; Hypo-activity; Worse rotarod performance than wild-type littermates. European Mouse Mutant Archive(EMMA)
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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