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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=597538592
Proper Citation: RRID:RGD_597538592
Description: Rattus norvegicus with name SD-Tg(SNCA)Nuber from RGD.
Species: Rattus norvegicus
Notes: For the generation of transgenic rats, the authors used a 190-kb fused AF163864 PAC/AC097478 BAC clone (Yamakado et al., 2012, PMID:22475625). It contained the entire human SNCA sequence (GenBank AF163864), with 30-kb upstream regulatory promoter sequences and a 45-kb flanking downstream region, cloned into pBACe3.6 vector as described previously. Transgenic rats were obtained by injecting the purified BAC fragment into fertilized Sprague–Dawley oocytes at a concentration of 1.5 µg/ml. A founder line displaying the highest integration number of BAC DNA construct was crossedbred to homozygosity.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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