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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=329849003
Proper Citation: RRID:RGD_329849003
Description: Rattus norvegicus with name LE-Pvalbem2(cre)Koba from RGD.
Species: Rattus norvegicus
Notes: A complex of Cas9 protein and gRNA was introduced into Iar:LE fertilized rat eggs by electroporation. Combi-CRISPR (Yoshimi K. et al.) was used to induce knock-in. Knock-in rats were selected and crossed with wild-type rats to establish this strain. Sequence features: the intron just before the fourth exon of the Pvalb gene (intron 3) is deleted by NHEJ after double-strand break by one base compared to the wild-type. ---ttggcgggccagaacctcagggg---(wild-type) ---ttggcgggccagaacc-cagggg---(knock-in) National BioResource Project for the Rat in Japan
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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