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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429707
Proper Citation: RRID:RGD_150429707
Description: Rattus norvegicus with name SD-Cyp2c11em1Nju from RGD.
Species: Rattus norvegicus
Notes: CRISPR/Cas9 system containing two pairs of single guide RNA (sgRNA) primers: sgRNA1 (forward primer: 59-GCTACTG- TAACTGACATGTT-39; reverse primer: 59-AACATGTCAGTTACAGTAGC- 39) and sgRNA2 (forward primer: 59-TCAAGGGTAAACTCAGACTG-39; reverse primer: 59-CAGTCTGAGTTTACCCTTGA-39), was injected to Sprague-Dawley rat one-cell embryos. The F0 pups were screened by genomic DNA sequencing to identify heterozygous CYP2C11+/2 founders.This mutant strain carries a two base pairs (GT) insertion into exon 6 of CYP2C11 and resulting in the knockout allele.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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