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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429615
Proper Citation: RRID:RGD_150429615
Description: Rattus norvegicus with name SD-Add3em1Mcwi/ Roman from RGD.
Species: Rattus norvegicus
Notes: ZFN system was used to Knock out the rat Add3 gene of Crl:SD rat embryos.The ZFN mRNA was injected into the pronucleus of fertilized SpragueDawley embryos and transferred to the oviduct of pseudopregnant females. PCR genotyping of tail biopsies confirmed a 14-bp deletion using forward primer 5'-GCCCCCATGAGTCACTACAC-3' and reverse primer 5'-GCTACAGGAAGCATCTCCTGTG-3'. Founders with Add3 deletion were backcrossed to the parental strain to generate heterozygous F1 rats. Heterozygous F1 siblings were then intercrossed to derive a homozygous KO line used
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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