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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=149735338
Proper Citation: RRID:RGD_149735338
Description: Rattus norvegicus with name SD-Wfs1em3Ptsn from RGD.
Species: Rattus norvegicus
Notes: Rat Wfs1 exon 5-specific zinc-finger nucleases (ZNFs) and microinjection-ready mRNA were injected to embryos harvested from female Sprague-Dawley rats (Crl: CD(SD) )rats. Thereafter, microinjected egg cells were transferred to the oviduct of pseudopregnant Sprague-Dawley recipients.Three different Wfs1 mutant rat lines were created: Wfs1em1 ( Wfs1-ex5-KO232), Wfs1em2 (Wfs1-ex5-KO266) and Wfs1em3 (Wfs1-ex5-INS244). Wfs1em3 rats carry a substitution in exon 5 of the Wfs1 gene, which is predicted to result in a substitution of LQK (aa 224-226) into YCMNTI in the WFS1 protein.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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