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URL: http://www.scienceexchange.com/facilities/custom-dna-constructs
Proper Citation: Custom DNA Constructs (RRID:SCR_013168)
Description: We provide plasmid cloning services. Our procedures allow cloning not only by using restriction enzymes but also in restriction-enzymes-independent ways. This allows sequence modification in areas where no suitable restriction sites are present. Examples for services offered: - Amplification of insert from one plasmid and cloning in another plasmid. - Addition of sequence to an existing plasmid, for example adding FLAG+myc tags, restriction sites, linkers, shRNA coding sequences, can add up to 100 bp in one cloning operation. - Introducing site directed mutations, can modify multiple bases within a 50 bp sequence in one cloning operation. - Deletion of sequences of any length from a plasmid. - Insertion of one gene fragment in to another gene, for example for making a chimeric protein. - Cloning an insert in to a plasmid while adding a tag at the 5'' or 3'' ends of the insert. - Cloning an insert while adding a polyglycine spacer to prevent structural effects of tags on your protein. - Any other sequence modifications by request.
Abbreviations: Custom DNA Constructs
Resource Type: access service resource, core facility, commercial organization, service resource
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The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant 2U24DK097771-09.
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