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URL: http://www.addgene.org/87399
Proper Citation: RRID:Addgene_87399
Insert Name: Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27899650
Vector Backbone Description: Vector Backbone:p426; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid has been found to exist as both a monomer and a dimer in our stock. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
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Source: Addgene
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