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URL: http://www.addgene.org/79433
Proper Citation: RRID:Addgene_79433
Insert Name: BIG1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15385626
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Vector Backbone:pcDNA4c; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: It is tricky to amplify the plasmid. After transformation of the plasmid into E. coli, not only the transformants grow very slowly (usually, it takes more than 20 h for the transformants to form visible colonies on ampicillin‐containing plates and colony size is smaller than usual one), but also their viability is drastically decreased during storage at 4C. Therefore, we routinely use fresh transformants for plasmid preparation. The plasmid yield decreases with the progress of the day after transformation. It is not recommended to make a pre-culture (~ 2ml) from a single colony for large scale culture. We strongly recommend that after transformation and recovery culture (for 1hr at 37C), bring all transformants to large scale culture (~ 200 ml) directly containing ampicillin.
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No rating or validation information has been found for pcDNA4c/His-Xpress-HA-hBIG1(E793K).
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Source: Addgene
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