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URL: http://www.addgene.org/40247
Proper Citation: RRID:Addgene_40247
Insert Name: attP Site
Organism: Synechococcus elongatus PCC 7942
Bacterial Resistance: Chloramphenicol and Spectinomycin
Defining Citation: PMID:16079339
Vector Backbone Description: Vector Backbone:pAM2314; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: our engineered NS1 Gateway “donor vector” the attP region of pDONR221 was amplified with primers H3140 and H3141, incorporating MluI and XhoI sites respectively and cloned into the NS1 vector pAM2314. the attP amplified region contains the upstream and downstream M13F and M13R annealing sites for sequencing inserts
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Source: Addgene
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