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URL: http://www.addgene.org/220537
Proper Citation: RRID:Addgene_220537
Bacterial Resistance: Ampicillin
Defining Citation: PMID:41372705
Vector Backbone Description: Vector Backbone:pAAV-Neo_CAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: pAAV-Neo_CAG is an empty AAVS1-targeting backbone used as a filler plasmid during genome editing. It carries a CAG-driven neomycin resistance cassette and is co-delivered with pAAV-Puro_siKD2.0 (Plasmid #220536) during nucleofection to enrich for biallelic AAVS1 integration. This plasmid does not express any shRNA or functional payload and serves only as a selection-compatible filler vector. Please visit https://dx.doi.org/10.17504/protocols.io.e6nvw4q29lmk/v1 for a detailed protocol for use.
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Source: Addgene
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