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URL: http://www.addgene.org/15023
Proper Citation: RRID:Addgene_15023
Insert Name: Ngn3 promoter
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11973276
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Comments: To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production. See Author's Map and article for more information.
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Source: Addgene
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