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URL: http://www.addgene.org/134449
Proper Citation: RRID:Addgene_134449
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32150173
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol.
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Source: Addgene
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