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URL: http://www.addgene.org/11651
Proper Citation: RRID:Addgene_11651
Insert Name: hUbiquitin C, GFP, tTR-KRAB, Tet-on
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16432520
Vector Backbone Description: Backbone Size:11480; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging. Note: There is another EcoRI site at 6235 that is not depicted in the author's map.
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Source: Addgene
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