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URL: http://www.addgene.org/100297
Proper Citation: RRID:Addgene_100297
Insert Name: PuroR.T2A.EGFP expression unit
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28939824
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al. 2017) for inducing homology-directed AAVS1 targeting. This donor plasmid has its targeting module flanked by the target site (TS) for gRNA.S1: 5’-CTGTCCCCTCCACCCCACAGTggggccactagggacaggattggTGACAGA-3’.
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Source: Addgene