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Antibody Name

*NOTICE: Multiple vendors found, please select your record:

EphB3 antibody
RRID:AB_381106 RRID Copied  
PDF Report How to cite
GeneTex Cat# GTX10617, RRID:AB_381106
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Antibody Information

URL: http://antibodyregistry.org/AB_381106

Proper Citation: GeneTex Cat# GTX10617, RRID:AB_381106

Target Antigen: EphB3 antibody

Host Organism: goat

Clonality: polyclonal

Comments: Discontinued; manufacturer recommendations: IgG; IgG ELISA: Use at a concentration of 0.5 - 1.0 µg/ml. The detection limit for recombinant mouse EphB3 is approx. 0.6 ng/well. WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EphB3 is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. The recombinant mouse EphB3 used for the testing of this antibody consists of the extracellular domain of mouse EphB3 (amino acids 1-530)1 fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB3 has Leu 23 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 82.8 kDa, but as a result of glycosylation, recombina

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Discontinued
Discontinued at GeneTex

View More at BIOMED RESOURCE WATCH

Data and Source Information
Authentication Plan

dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.