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URL: http://antibodyregistry.org/AB_2892150
Proper Citation: (Bertram Jacobs Arizona State University Cat# 1796, RRID:AB_2892150)
Target Antigen: E3L
Host Organism: rabbit
Clonality: polyclonal
Comments: "Electrophoretically purified ~25 was used to immunize a rabbit by the method of Boulard and Lecroisey (1982). Extracts of ara c-treated vaccinia-infected L cells prepared at 20 hpi were used as a source of ~25. The protein was isolated by poly(rl). poly(rC)-Sepharose adsorption and SDS/PAGE. The gel was soaked in 100 mM KCI at 4” to visualize protein bands. The M, = 25,000 band was excised, and the gel slice was washed in water, crushed in PBS, and mixed with an equal volume of Freund’s complete (for primary immunization), or incomplete (for secondary immunizations) adjuvant. This mixture was used for subcutaneous immunization of a rabbit. A total of approximately 3 pg protein was used in each immunization."
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Source: Antibody Registry
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